The Future of Remediation: Rhamnolipid Biosurfactants

Traditionally, the remediation of harmful substances entails saturating the environment with chemicals that solve the problem locally but initiate contamination elsewhere. No matter how safe the solution used in remediation purports to be, no environment responds well to excessive quantities of a foreign element. Using naturally derived and environmentally friendly rhamnolipid biosurfactant used in tailored proportions, saves time and money, and prevents the local environment from becoming saturated with unnatural quantities of remediation chemicals.



Precise calculation of ratios of Mono-rhamnolipid to Di-rhamnolipid, their carriers, and their dilutions for specific applications



We can apply the natural and non-toxic applications to deter the buildup of microbes and eliminate them before they cause problems.

*Rhamnolipid can be obtained through third parties. Currently working down inventory.*

What is rhamnolipid?

Rhamnolipids are produced naturally in the environment. The bacteria pseudomonas aeruginosa is found everywhere from indoors to outdoors, including but not limited to, counter tops in our kitchen, lakes, streams and in soil. The chemical rhamnolipid is secreted by the bacteria to protect itself and survive. Rhamnolipid is produced by a fermentation process similar to those used to produce beer or other fermented products. Unique strains from our library of bacteria are added to a fermentation tank and provided a nutrient source, such as an oil. Using unique processes, properly controlled conditions, and manufacturing trade secrets, low cost rhamnolipids are produced in industrial quantities. The rhamnolipids are extracted, processed to remove any residual bacteria, purified, and the resultant mixture diluted into a final product. The amount and mixture of rhamnolipid material in the final solution can be precisely controlled. The rhamnolipid mixture can be further refined to produce certain foaming characteristics and mixed according to specific agricultural applications such as a basic wetting agent.

Pseudomonas Aeruginosa - © Dennis Kunkel Microscopy

Biosurfactants vs Synthetic Surfactants

Rhamnolipid biosurfactants are made from living organisms. They are found in the environment and are non-toxic. Synthetic surfactants are man-made and mostly derived from petroleum, “oil-based” products. They have been widely used in industry for many years because they were relatively inexpensive. Synthetic surfactants have serious ecological impacts, both by depleting a non-renewal resource and by leaving non-biodegradable and harmful byproducts. They are destroying the environment.


United States Environmental Protection Agency

ai Our rhamnolipid, RhamnoWash 10™, is a naturally derived biosurfactant, consisting of lipid and rhamnose sugar molecules. On August 3, 2015, the U.S. Environmental Protection Agency (EPA) issued a letter to us regarding the listing of RhamnoWash 10™ as a Surface Washing Agent on National Oil and Hazardous Substance Pollution Contingency Plan (NCP) Product Schedule maintained by the USEPA. In order to list RhamnoWash 10™, technical product data was submitted to the USEPA pursuant to 40 CFR 300.915(b).

In 2004, The EPA issued a letter stating that rhamnolipid was accepted as a spray to kill pests on agriculture products in the United States. It also noted that rhamnolipid was also registered “safe” to be ingested by humans at very low concentrations.

Florida Department of Environmental Protection


On February 24, 2014, we were granted approval for rhamnolipid wastewater and soil washing applications. The letter indicates that the Division of Waste Management accepts Rhamnolipid Aqueous Solution for in situ bioremediation of petroleum hydrocarbons and metals in groundwater and soil.


Helen Zhang, Professor at Memorial University, has successfully tested our
biosurfactants via an adsorption experiment for investigating cobalt removal from soil.

"Adsorption Behaviour of Cobalt onto Saline Soil with/without a Biosurfactant: Kinetic and Isotherm Studies"

Publication Manuscript

Canada Research Chair (Coastal Environmental Engineering)
Associate Professor, Civil Engineering
Faculty of Engineering and Applied Science

"When applying rhamnolipid, by increasing the initial concentration of Co, the remaining Co in the solution increased considerably at any initial concentration of rhamnolipid. The high value range of surface tension of solutions (60-70 mN/m) shows that rhamnnolipid has a high tendency of adsorption to the soil surface."